Caspase 9 (CASP9) Rabbit Polyclonal Antibody

CAT#: TA327390

Rabbit anti-CASP9 Polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using CASP9 antibody.

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  Immunohistochemistry of paraffin-embedded rat liver using CASP9 antibody at dilution of 1:100 (40x lens).

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  Immunohistochemistry of paraffin-embedded human colon carcinoma using CASP9 antibody at dilution of 1:100 (40x lens).

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  Immunohistochemistry of paraffin-embedded human stomach using CASP9 antibody at dilution of 1:100 (40x lens).

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  Immunohistochemistry of paraffin-embedded mouse brain using CASP9 antibody at dilution of 1:100 (40x lens).

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  Immunofluorescence analysis of A549 cell using CASP9 antibody. Blue: DAPI for nuclear staining

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Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500 - 1:2000;IHC 1:100 - 1:200;IF 1:50 - 1:100
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human CASP9
• Formulation: Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 46 kDa
• Gene Name: caspase 9
• Database Link:
  NP_001220
  Entrez Gene 12371 MouseEntrez Gene 58918 RatEntrez Gene 842 Human
  UniProt ID P55211
• Background: Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family. Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9/Apaf 1. Apaf-1 mediated activation of caspase-9 involves instrinsic proteolytic processing resulting in cleavage at Asp315 and producing a p35 subunit. Another cleavage occurs at Asp330 producing a p37 subunit that can serve to amplify the apoptotic response. Cleaved caspase-9 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, which leads to apoptosis.
• Synonyms: APAF-3; APAF3; ICE-LAP6; MCH6; PPP1R56

Reference Data

• Protein Families: Druggable Genome, Protease, Stem cell - Pluripotency
• Protein Pathways: Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Colorectal cancer, Endometrial cancer, Huntington's disease, Non-small cell lung cancer, p53 signaling pathway, Pancreatic cancer, Parkinson's disease, Pathways in cancer, Prostate cancer, Small cell lung cancer, VEGF signaling pathway, Viral myocarditis