SPI1 Rabbit Monoclonal Antibody [Clone ID: EPR3158Y]

CAT#: TA327731

Rabbit Monoclonal PU.1 Antibody

Product Images

Immunohistochemistry staining of Paraffin Tonsil tissue by PU.1 antibody (dilution: 1:50 - 1:200; visualization of staining: Nuclear)

Specifications

Product Data

• Clone Name: EPR3158Y
• Applications: IHC
• Recommended Dilution: IHC: 1:50 - 1:200
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Formulation: The antibody is diluted in tris buffered saline, pH 7.3-7.7, with 1% BSA and <1% sodium azide.
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Spi-1 proto-oncogene
• Database Link:
  NP_001074016
  Entrez Gene 6688 Human
  UniProt ID P17947
• Synonyms: OF; PU.1; SFPI1; SPI-1; SPI-A
• Note: PU.1 is a transcription factor that has been shown to be important for normal B-cell development. PU.1 belongs to the Ets family of transcription factors. It is expressed in the myeloid lineage and in immature as well as mature B-lymphocytes, with the exception of plasma cells. PU.1 is essential during early B-cell differentiation. The absence of PU.1 results in total block of B-cell development at the pre-pro stage. PU.1 is expressed in germinal center B-cells and mantle B-cells. Various lymphomas are also positive for anti-PU.1 including the following: B-chronic lymphocytic leukemia, mantle cell lymphoma, follicular lymphoma, marginal zone lymphoma, diffuse large cell lymphoma, diffuse large B-cell lymphoma, and nodular lymphocyte predominant Hodgkin lymphoma. Torlakovic et al. have demonstrated a quantitative positive association between a high level of expression of germinal center antigens (including PU.1) and a longer overall survival and progression-free survival in the case of follicular lymphoma.

Reference Data

• Protein Families: Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Pathways in cancer