Histone H2A.X (H2AFX) Mouse Monoclonal Antibody [Clone ID: 2F3]

CAT#: TA327941

Mouse Monoclonal anti-H2A.X Phospho (Ser139) Antibody

Product Images

Untreated (Lane 1) and staurosporine-treated (Lane 2) Jurkat nuclear extract, untreated (Lane 3) and UV treated (Lane 4) Jurkat cell extract were western blotted using anti-phospho-H2A.X (Ser 139) antibody, clone: 2F3.

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  Untreated Hela cells (Panel A), or overnight nocodazole treated Hela cells (Panel B) stained with purified mouse monoclonal antibody against Ser139 phosphorylated H2A.X, followed by Rhodamine Red-X conjugated Donkey anti-mouse IgG and DAPI.

Specifications

Product Data

• Clone Name: 2F3
• Applications: IF, WB
• Recommended Dilution: WB, IHC, ICFC, IF
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1, kappa
• Clonality: Monoclonal
• Immunogen: Modified peptide
• Formulation: This H2A.X antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.
• Concentration: lot specific
• Purification: The antibody was purified by affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 14 kD
• Gene Name: H2A histone family member X
• Database Link:
  NP_002096
  Entrez Gene 15270 MouseEntrez Gene 3014 Human
  UniProt ID P16104
• Background: H2A.X is a 14 kD basal histone and a member of the H2 histone family. This nuclear protein is synthesized in the G1 and S phase of the cell cycle and is known to be important for recombination between immunoglobulin switch regions. H2A.X becomes phosphorylated on serine 139 after double-stranded DNA breaks. Phosphorylated H2A.X promotes DNA repair and maintains genomic stability. The 2F3 monoclonal antibody reacts with phosphorylated human H2A.X (Ser139) and has been shown to be useful for Western blotting and immunofluorescence.
• Synonyms: H2A; H2A.X; H2AX; X

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Systemic lupus erythematosus