H3FT (HIST3H3) Rat Monoclonal Antibody [Clone ID: HTA28]

CAT#: TA327973

Rat Monoclonal anti-Histone H3 Phospho (Ser28) Antibody

Product Images

Western blot analysis of extracts from untreated Hela cells (lane 1) or overnight nocodazole-treated Hela cells (lane 2), using anti-phospho-Histone H3 (Ser28), clone HTA28.

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  Untreated HeLa (Panel A) and overnight nocodazoled-treated HeLa (Panel B) were stained with purified rat monoclonal antibody against phospho-H3 (Ser28) (clone HTA28), followed by Alexa Fluor® 488 anti-alpha-tubulin, DyLight™ 594 goat anti-rat-IgG and DAPI.

Specifications

Product Data

• Clone Name: HTA28
• Applications: IF, WB
• Recommended Dilution: WB, IP, ICFC, IF, CyTOF
• Reactivities: Human
• Host: Rat
• Isotype: IgG2a, kappa
• Clonality: Monoclonal
• Immunogen: Synthetic peptide conjugated to KLH, corresponding to amino acids 23-35 of Human Histone H3.
• Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.
• Concentration: lot specific
• Purification: The antibody was purified by affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: histone cluster 3, H3
• Database Link:
  NP_003484
  Entrez Gene 8290 Human
  UniProt ID Q16695
• Background: H3 is a core component of the nucleosome that serves to wrap and compact DNA into chromatin.Histones therefore, limit the accessibility of DNA, providing mechanisms for transcription regulation, DNA repair and replication and chromosomal stability.During mitosis, H3 is phosphorylated at serine 28.This phosphorylation coincides with chromosome condensation initiated at prophase and disappears at late anaphase H3 has been demonstrated to be phosphorylated by the action of MLTK-α (mixed linage kinase-like mitogen activated protein triple kinase α) in response to ultraviolet B light and epidermal growth factor, as well as Aurora-B during mitosis.
• Synonyms: g; H3; H3.4; H3FT; H3t

Reference Data

• Protein Pathways: Systemic lupus erythematosus