Adenosine A3 Receptor (ADORA3) Rabbit Polyclonal Antibody

CAT#: TA328606

Rabbit Polyclonal Anti-A3 Adenosine Receptor

Product Images

Western blot analysis of Malme-3M cell lysate: 1. Anti-A3 Adenosine Receptor antibody, (1:200). 2. Anti-A3 Adenosine Receptor antibody, preincubated with the control peptide antigen.

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  Expression of A3 Adenosine Receptor in rat lung. Immunohistochemical staining of paraffin-embedded rat lung sections using Anti-A3 Adenosine Receptor antibody, (1:50). Staining is specific for respiratory epithelial cells of the bronchiole (arrows) and pneumonocytes in the alveolar wall (a). Hematoxilin is used as the counterstain.

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  Expression of A3 Adenosine Receptor in a human melanoma cell line. Immunocytochemical staining of paraformaldehyde-fixed and permeabilized human melanoma cells (A2058). A, D. Cells were stained using Anti-A3 Adenosine Receptor antibody, (1:100), followed by goat anti-rabbit AlexaFluor-555 secondary antibody. B, E. Nuclear fluorescence staining of cells using the membrane-permeable DNA dye Hoechst 33342. C. Merged image of panels A and B. F. Merged image of panels D and E.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:200-1:2000; IHC: 1:100-1:3000
• Reactivities: Human, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide (C)KETGAFYGREFKTAK, corresponding to amino acid residues 216-230 of human A3AR. 3rd intracellular loop.
• Formulation: Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
• Purification: Affinity purified on immobilized antigen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: adenosine A3 receptor
• Database Link:
  NP_000668
  Entrez Gene 25370 RatEntrez Gene 140 Human
  UniProt ID P0DMS8
• Background: Adenosine is an endogenous nucleoside generated locally in tissues under conditions of hypoxia, ischemia, or inflammation. It modulates a variety of physiological functions in many tissues including brain and heart. Adenosine exerts its action via four specific adenosine receptors (also named P1 purinergic receptors): A1-Adenosine receptor (A1AR), A2A-Adenosine receptor (A2AAR), A2B-Adenosine receptor (A2BAR), and A3-Adenosine receptor (A3AR). The various adenosine receptors can be distinguished on the basis of their distinct molecular structures, distinct tissue distributions, and differential selectivity for adenosine analogs. However, all are integral membrane proteins and members of the G Protein-Coupled Receptor superfamily. They share a common structure of seven putative transmembrane domains, an extracellular amino terminus, a cytoplasmic carboxyl terminus, and a third intracellular loop that is important for binding G proteins. Expression of A3AR has been reported in brain, kidney, liver, and heart. It plays a role in modulation of cerebral ischemia, asthma, and cell growth. In addition, recent studies have established a cardioprotective role for A3AR. Expression of A3AR was reported to be elevated in cancerous tissues as well as in auto-immune inflammatory diseases. A patent has been filed for the use of A3AR and A3AR agonist as a diagnostic marker for therapeutic treatments of cancer and other diseases.
• Synonyms: A3AR

Reference Data

• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction