Adenosine A1 Receptor (ADORA1) Rabbit Polyclonal Antibody

CAT#: TA328694

Rabbit Polyclonal Anti-A1 Adenosine Receptor

Product Images

Western blot analysis of rat brain (lanes 1, 3) and rat kidney (lanes 2, 4) lysates: 1, 2. Anti-A1 Adenosine Receptor antibody, (1:200). 3, 4. Anti-A1 Adenosine Receptor antibody, preincubated with the control peptide antigen.

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  Expression of A1 Adenosine Receptor in rat cortex Immunohistochemical staining of rat cortex frozen section using Anti-A1 Adenosine Receptor antibody. A. A1 Adenosine Receptor (green) appears in neurons (triangles) and in astrocytes (arrows). B. Parvalbumin staining (red) appears in cortical interneurons. C. Confocal merge of images demonstrates the existence of A1 Adenosine Receptor in a subset of cortical interneurons and astrocytes.

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  Expression of A1 Adenosine Receptor in rat medial septum. Immunohistochemical staining of rat medial septum frozen section using Anti-A1 Adenosine Receptor antibody. A. A1 Adenosine Receptor (green) appears in neurons (right pointing triangles). B. Parvalbumin staining (red) appears in medial septal neurons. C. Confocal merge of demonstrates expression of A1 Adenosine Receptor in a subset of medial septal neurons.

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  Expression of A1 Adenosine Receptor in rat DRG primary culture. Immunocytochemical staining of paraformaldehyde-fixed and permeabilized rat dorsal root ganglion (DRG) primary culture. A. Staining of DRG cells with Anti-A1 Adenosine Receptor antibody, (1:100), followed by goat anti-rabbit AlexaFluor-555 secondary antibody. B. Nuclear staining of cells using the cell-permeable dye Hoechst 33342. C. Merged images of A and B. F. Merged images of D and E. Magnification: A-C: x20 D-F: x100

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:200-1:2000; IHC: 1:100-1:3000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide (C)KKVSASSGDPQKYYGKE, corresponding to amino acid residues 213-229 of human A1AR. 3rd intracellular loop.
• Formulation: Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
• Purification: Affinity purified on immobilized antigen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: adenosine A1 receptor
• Database Link:
  NP_001041695
  Entrez Gene 11539 MouseEntrez Gene 29290 RatEntrez Gene 134 Human
  UniProt ID P30542
• Background: Adenosine is an endogenous nucleoside generated locally in tissues under conditions of hypoxia, ischemia, or inflammation. It modulates a variety of physiological functions in many tissues including brain and heart.It exerts its action via four specific adenosine receptors (also named P1 purinergic receptors): A1-Adenosine Receptor (A1AR), A2A-Adenosine Receptor (A2AAR), A2B-Adenosine Receptor (A2BAR), and A3-Adenosine Receptor (A3AR). All are integral membrane proteins and members of the G Protein-Coupled Receptor superfamily. They share a common structure of seven putative transmembrane domains, an extracellular amino terminus, a cytoplasmic carboxyl terminus, and a third intracellular loop that is important in binding G proteins. The various adenosine receptors can be distinguished on the basis of their differential selectivity for adenosine analogs. A1AR is widely distributed and has been well characterized. High expression of A1AR is found in brain (mainly in the cortex, cerebellum, and hippocampus), dorsal horn of the spinal cord, adrenal gland, and atria, and to a lower extent in several other tissues including adipose tissue, colon, and kidney. A1AR modulates the activity of several ion channels. Activation of A1AR (by adenosine, its major agonist) inhibits N-type Ca2+ channels via a voltage-dependent, pertussis toxin (PTX)-sensitive pathway in neurons of the rat major pelvic ganglia (MPG). Since A1AR is the most prominent adenosine receptor in adipocytes, it has become a natural target for research on obesity, which is a major health problem. A possible role in cell proliferation and carcinogenesis has also been suggested for A1AR.
• Synonyms: RDC7

Reference Data

• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction