Adra2a Rabbit Polyclonal Antibody

CAT#: TA328713

Rabbit Polyclonal Anti-alpha2A-Adrenoceptor (extracellular)

Product Images

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates: 1, 2. Anti-a2A-Adrenoceptor (extracellular) antibody, (1:200). 3, 4. Anti-a2A-Adrenoceptor (extracellular) antibody, preincubated with the control peptide antigen.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:200-1:2000
• Reactivities: Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide (C)DAGNSSWNGTEAPG, corresponding to amino acid residues 7-20 of rat a2A-Adrenoceptor. Extracellular, N-terminus.
• Formulation: Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
• Purification: Affinity purified on immobilized antigen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: adrenoceptor alpha 2A
• Database Link:
  NP_036871
  Entrez Gene 11551 MouseEntrez Gene 25083 Rat
  UniProt ID P22909
• Background: Adrenoceptors (also called Adrenergic receptors) are the receptors for the catecholamines adrenaline and noradrenaline (called epinephrine and norepinephrine in the United States).Adrenaline and noradrenaline play important roles in the control of blood pressure, myocardial contractile rate and force, airway reactivity, and a variety of metabolic and central nervous system functions.The Adrenoceptors are members of the G-Protein Coupled Receptor (GPCR) superfamily of membrane proteins. They share a common structure of seven putative transmembrane domains, an extracellular N-terminus, and a cytoplasmic C- terminus.The Adrenoceptors are divided into three types: a1, a2 and Ã?-Adrenoceptors. Each type is further divided into at least three subtypes: a1A, a1B, a1D, a2A, a2B, a2C, Ã?1, Ã?2, Ã?3. The Adrenoceptors are expressed in nearly all peripheral tissues and in the central nervous system.In general, the pharmacological properties of each subtype are quite homogenous across different species. However, this is not the case for the a2A subtype which was first isolated from human platelets3. This subtype shows different pharmacological properties from that of mouse and rat. For this reason, until molecular techniques significantly advanced, it was believed that human a2A and that of mouse and rat (termed a2D) were two different subtypes. Today, it is accepted that these two subtypes are in fact one gene product and is generally termed a2A. One of the main differences between this subtype from the different organisms is its affinity for yohimbine and rauwolscine; rat and mouse a2A displays less affinity compared to human.
• Synonyms: ADRA2; ADRA2R; ADRAR; Alpha-2AAR; ALPHA2AAR; ZNF32