Cacna2d4 Rabbit Polyclonal Antibody

CAT#: TA328762

Rabbit Polyclonal Anti-CaVAlpha2Delta4 (extracellular)

Product Images

Western blot analysis of rat DRG (lanes 1 and 3) and PC12 cells (lanes 2 and 4): 1-2. Anti-Cava2d4 (extracellular) antibody, (1:200). 3-4. Anti-Cava2d4 (extracellular) antibody preincubated with the control antigen.

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  Expression of Cava2d4 in rat DRG. Immunohistochemical staining of rat DRG frozen section using Anti-Cava2d4 (extracellular) antibody, (1:100). A. Cava2d4 is expressed in DRG neurons. B. Nuclear staining using Hoechst 33342 as the counterstain. C. Merged images of A and B.

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  Expression of Cava2d4 in rat C6 cells. Immunocytochemical staining of intact living rat brain glioma (C6) cells. A. Extracellular staining of cells with Anti-Cava2d4 (extracellular) antibody, (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Nuclear staining of cells using the cell-permeable dye Hoechst 33342 (blue). C. Merged images of A and B.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:200-1:2000; IHC: 1:100-1:3000
• Reactivities: Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide (C)SERPQEMGRLLGEADG, corresponding to amino acid residues 881-896 of mouse Cava2d4. Extracellular.
• Formulation: Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
• Purification: Affinity purified on immobilized antigen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: calcium channel, voltage-dependent, alpha 2/delta subunit 4
• Database Link:
  NP_001028554
  Entrez Gene 312668 RatEntrez Gene 319734 Mouse
  UniProt ID Q5RJF7
• Background: Voltage-gated Ca2+ (CaV) channels are ubiquitously expressed and function as Ca2+ conducting pores in the plasma membrane. Based on their electrophysiological and pharmacological properties, Ca2+ channels have traditionally been classified into L, T, N, P, Q, and R types. L-type calcium channels are heteromultimers composed of four independently encoded proteins, the pore-forming a1 subunit, which triggers Ca2+ flow across the membrane, and the auxiliary subunits a2d, ?, and Ã?. The Ca2+ channel a2d subunit is a heavily glycosylated protein that is encoded by a single gene and post-translationally cleaved to yield a2 and d subunits linked by a disulfide bond with a single transmembrane segment.The a2d subunit regulates many functional aspects of Ca2+ channels, such as gating, regulating voltage dependent kinetics, and increasing functional channel density on the plasma membrane. There are four proteins that comprise CaVa2d: CaVa2d1, CaVa2d2, CaVa2d3 and CaVa2d4. CaVa2d4 participates in the regulation of membrane expression of CaV channels. It is predominantly expressed in certain types of endocrine cells. It is also detected in the erythroblasts in the fetal liver, in the cells of the zona reticularis of the adrenal gland and in the basophiles of the pituitary. Defects in CaVa2d4 are the cause of retinal cone dystrophy 4 (RCD4). RCD4 is characterized by minimal symptoms except for slowly progressive reduction in visual acuity.
• Synonyms: RCD4
• Note: This antibody was tested in live cell imaging. Please see IF/ICC data for detail.