Slc18a3 Rabbit Polyclonal Antibody

CAT#: TA328797

Rabbit Polyclonal Anti-Vesicular Acetylcholine Transporter

Product Images

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates: 1-2. Anti-Vesicular Acetylcholine Transporter antibody, (1:200). 3-4. Anti-Vesicular Acetylcholine Transporter antibody, preincubated with the control peptide antigen.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:200-1:2000
• Reactivities: Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide (C)DAVRLREVQGKDGGE, corresponding to amino acid residues 495-509 of rat vesicular acetylcholine transporter. Cytoplasmic, C-terminus.
• Formulation: Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
• Purification: Affinity purified on immobilized antigen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: solute carrier family 18 member A3
• Database Link:
  NP_113851
  Entrez Gene 20508 MouseEntrez Gene 60422 Rat
  UniProt ID Q62666
• Background: In cholinergic neurons, acetylcholine is synthesized from choline and acetyl-coenzyme A (acetyl-CoA). Vesicular acetylcholine transporter (VAChT) transports acetylcholine from the cytoplasm to the lumen of synaptic vesicles where following depolarization, acetylcholine is released in the synaptic cleft and activates muscarinic and nicotinic receptors located on postsynaptic membranes. Acetylcholine released in the synaptic cleft is rapidly hydrolyzed into choline and acetate. Since choline is not de novo synthesized and thereby only made available through diet uptake, choline is recycled back into the cells in order to regenerate acetylcholine. VAChT is a member of the SLC18 family of transporters. It has 12 membrane spanning domains, with extracellular N- and C-terminal tails. The transporter exchanges cytoplasmic acetylcholine for two vesicular protons. VAChT is a selective marker of cholinergic neurons and could be used therefore to determine the loss of cholinergic neurons in Alzheimerâ??s or Alzheimerâ??s related disease. VAChT knockout mice die after birth. They display a lack of stimulated release of acetylcholine and an underdeveloped neuromuscular junction.
• Synonyms: MGC12716; VACHT