Gabrd Rabbit Polyclonal Antibody

CAT#: TA328816

Rabbit Polyclonal Anti-GABA(A) delta Receptor (extracellular)

Product Images

Western blot analysis of rat brain (lanes 1, and 4), rat cerebellum (lanes 2 and 5) and mouse brain (lanes 3 and 6): 1-3. Anti-GABA(A) d Receptor (extracellular) antibody, (1:200). 4-6. Anti-GABA(A) d Receptor (extracellular) antibody, preincubated with the control peptide antigen.

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  Expression of GABA(A) d receptor in rat cerebellum. Immunohistochemical staining of GABA(A) d receptor in rat cerebellum using Anti-GABA(A) d Receptor (extracellular) antibody. GABA(A) d receptor (red) appears exclusively in the granule layer (G). Above and below the granule layer is the molecular layer (M). DAPI is used as the counterstain (blue).

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1:200-1:2000; IHC: 1:100-1:3000
• Reactivities: Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide (C)HHGARAMNDIGDYVGSN, corresponding to amino acid residues 19-35 of rat GABA(A)d .
• Formulation: Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
• Purification: Affinity purified on immobilized antigen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: gamma-aminobutyric acid type A receptor delta subunit
• Database Link:
  NP_058985
  Entrez Gene 14403 MouseEntrez Gene 29689 Rat
  UniProt ID P18506
• Background: GABA (?-aminobutyric acid) is the major inhibitory neurotransmitter in the brain. Its production, release, reuptake, and metabolism all occur in the nervous system.The GABA transmitter interacts with two major types of receptors: ionotropic GABAA receptors (GABAAR) and metabotropic receptors (GABABR). GABAARs belong to the ligand-gated ion channel superfamily. GABA inhibits the activity of signal-receiving neurons by interacting with the GABAA receptor on these cells. Binding of GABA to its GABAA receptor results in conformational changes that open a Cl- channel, producing an increase in membrane conductance that results in inhibition of neural activity.GABAARs are heteropentamers, in which all five subunits contribute to the pore formation. To date, eight subunit isoforms have been cloned: a, Ã?, ?, d, e, p, ?, and ?. Six a subunit isoforms have been found to exist in mammals (a1-a6). In most cases, native GABAA receptors consist of 2a, 2Ã?, and 1? subunits. The a subunit is the most common and is expressed ubiquitously. It determines the affinity of GABAARs for allosteric ligands.Each subtype has a unique regional expression in the brain, and individual neurons often express multiple subtypes. For example, the a4 subunit is detected in the hippocampus, cortex, olfactory bulb and in the basal forebrain.
• Synonyms: EIG10; EJM7; GEFSP5; MGC45284