Chrnb2 Rabbit Polyclonal Antibody

CAT#: TA328887

Rabbit Polyclonal Anti-Nicotinic Acetylcholine Receptor beta2 (extracellular)

Product Images

Western blot analysis of SH-SY5Y human neuroblastoma cell lysate (lanes 1 and 4) and rat (lanes 2 and 5) and mouse (lanes 3 and 6) brain lysate: 1-3. Anti-Nicotinic Acetylcholine Receptor β2 (extracellular) antibody, (1:200). 4-6. Anti-Nicotinic Acetylcholine Receptor β2 (extracellular) antibody, preincubated with the control peptide antigen.

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  Expression of Nicotinic Acetylcholine Receptor β2 in rat PC12 cells. Immunocytochemical staining of live intact rat pheochromocytoma PC12 cells. A. Cells were stained with Anti-Nicotinic Acetylcholine Receptor β2 (extracellular) antibody, (1:100), followed by goat anti-rabbit-AlexaFluor- 594 secondary antibody (red). B. Cell nuclei were visualized using Hoechst 33342 (blue). C. Live view of the cells.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 1:200-1:2000; FC: 1:50-1:600
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide (C)EDFDNMKKVRLP, corresponding to amino acid residues 96-107 of rat nAChRÃ?2. Extracellular, N-terminus.
• Formulation: Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
• Purification: Affinity purified on immobilized antigen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: cholinergic receptor nicotinic beta 2 subunit
• Database Link:
  NP_062170
  Entrez Gene 1141 HumanEntrez Gene 11444 MouseEntrez Gene 54239 Rat
  UniProt ID P12390
• Background: Acetylcholine, released by cholinergic neurons, activates two groups of acetylcholine receptors (AChRs); muscarinic AChRs (mAChRs) which belong to the superfamily of G-protein coupled receptors (GPCRs) and nicotinic AChRs (nAChRs) which belong to the ligand-gated ion channel superfamily. nAChRs also respond to nicotine, hence their name.To date, 17 different but related subunits of nAChRs have been identified and cloned. They consist of a subunits (a1-10), which is responsible for the binding of ligands. In fact, this subunit includes a Cys-loop in the first extracellular domain that is required for agonist binding. The other subunits responsible for making up the active receptor are the Ã? (Ã?1-4), ?, d and e subunits. Structurally, all subunits have the following: a conserved large extracellular N-terminal domain, 3 conserved transmembrane domains, a variable cytoplasmic loop and a fourth transmembrane domain with a short extracellular C-terminal domain. An active nAChR is generally a heteropentamer of these various subunits organized around a central pore.While most Ã? subunits are neuronal, the Ã?1 subunit forms functional receptors along with other subunits in the muscle. The diversity of these receptors and their functional organization gives rise to unique properties and functions. The a4Ã?2 receptor composition makes up a high affinity nicotinic receptor. In fact, its upregulation (mainly expressed by the increase of functional receptors at the membrane and not expression per se) is responsible for the increased appearance of binding sites following nicotine administration.Animal studies have shown that nAChR-related mechanisms are involved in attention function. Indeed a4Ã?2 nAChR seems to also be involved in attention-deficit hyperactivity disorder (ADHD), a disease distinguished by a lack of attention, distractibility and hyperactivity. The a4Ã?2 and a7 nAChRs appear to be critical in rats for attention and working memory. Also, a a4Ã?2 specific agonist was shown to reduce impulsivity, hyperactivity and attention deficits in adults with ADHD5. This same receptor subtype may also be involved in Parkinsonâ??s disease (PD) as smoking and a4Ã?2 nAChR agonists show beneficial effects in PD.
• Synonyms: EFNL3; nAChRB2
• Note: This antibody was tested in live cell imaging. Please see IF/ICC data for detail.