Kcnk13 Rabbit Polyclonal Antibody

CAT#: TA328974

Rabbit polyclonal Anti-K2P13.1 (THIK-1) (extracellular)

Product Images

Western blot analysis of rat kidney membranes: 1. Anti-K2P13.1 (THIK-1) (extracellular) antibody, (1:200). 2. Anti-K2P13.1 (THIK-1) (extracellular) antibody, preincubated with the control peptide antigen.

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  Western blot analysis of mouse heart membranes: 1. Anti-K2P13.1 (THIK-1) (extracellular) antibody (1:200). 2. Anti-K2P13.1 (THIK-1) (extracellular) antibody, preincubated with the control peptide antigen.

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  Expression of K2P13.1 in rat kidney. Immunohistochemical staining in paraffin-embedded sections of rat kidney using Anti-K2P13.1 (THIK-1) (extracellular) antibody, (1:50). Staining (brown color) is specific for renal tubules while both glomeruli (G) and arterial walls are negative (red arrows). Following Tris-EDTA treatment for antigen retrieval, slides were incubated overnight at 4°C. Hematoxylin is used as the counterstain.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1:200-1:2000; IHC: 1:100-1:3000
• Reactivities: Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide (C)EERLANFSRGHNLSRE, corresponding to amino acid residues 54-69 of rat K2P13.1. 1st extracellular loop..
• Formulation: Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
• Purification: Affinity purified on immobilized peptide.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: potassium two pore domain channel subfamily K member 13
• Database Link:
  NP_071629
  Entrez Gene 217826 MouseEntrez Gene 64120 Rat
  UniProt ID Q9ERS0
• Background: K2P13.1 (also named Tandem Pore Domain Halotane Inhibited K+ channel, THIK -1or KCNK13) is a member of the 2-pore (2P) domain K+ channels family that at the moment includes 15 members. These channels show little time or voltage dependence and are considered to be â??leakâ? or â??backgroundâ? K+ channels, thereby generating background currents which help set the membrane resting potential and cell excitation.The K2P channels have a signature topology that includes four transmembrane domains and two pore domains with intracellular N- and C termini. It has been proposed that the functional channel unit is a dimer.K2P channels are regulated by diverse physical and chemical stimuli including temperature, pH, mechanical stretch, inhalation anesthetics, etc. but are insensitive to classical K+ channel blockers.The K2P13.1 channel is activated by arachidonic acid and inhibited by the volatile anesthetic halothane. The channel is relatively widely expressed in peripheral tissues such as kidney, heart, lung and liver but it is also expressed in brain.The physiological function of the K2P13.1 channel is not clear but an involvement in the physiological response to reduced oxygen levels (hypoxia) has been proposed.
• Synonyms: K2p13.1; THIK-1; THIK1