Scn2b Rabbit Polyclonal Antibody

CAT#: TA329032

Rabbit polyclonal Anti-Nabeta2

Product Images

Western blot analysis of rat brain membranes under reducing (lanes 1 and 2) or non-reducing (lanes 3 and 4) conditions:* 1, 3. Anti-Navβ2 antibody, (1:200). 2, 4. Anti-Navβ2 antibody, preincubated with the control peptide antigen. * Navβ2 is disulfide-linked to the α subunit of voltage-gated Na+ channels (Schmidt, J.W., and Catterall, W.A. (1986) Cell 46, 437.)

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  Expression of Navβ2 in mouse cerebellum. Immunohistochemical staining of mouse cerebellum using Anti-Navβ2 antibody. A. Navβ2 channel (red) appears in Purkinje cells (vertical arrows) and in small cells in the molecular layer (horizontal arrows, Mol). B. Staining of Purkinje nerve cells with mouse anti-calcium binding protein (CBD28k, green) demonstrates the restriction of Navβ2 to cell bodies. C, Confocal merge of Navβ2 and CBD28k.

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  Expression of Navβ2 in rat cerebellum. Immunohistochemical staining of rat cerebellum using Anti-Navβ2 antibody. A. Navβ2 channel (red) appears in Purkinje cells (horizontal arrows) and is distributed diffusely in the molecular layer (Mol). B. Staining of Purkinje nerve cells with mouse anti-calcium binding protein (CBD28k, green) demonstrates the restriction of Navβ2 to cell bodies. C. Confocal merge of Navβ2 and CBD28k.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1:200-1:2000; IHC: 1:100-1:3000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide (C)KTEEEGKTDGEGNAEDGAK, corresponding to amino acid residues 197-215 of rat ?²2 subunit of voltage-gated Na+ channels. Intracellular, C-terminus.
• Formulation: Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
• Purification: Affinity purified on immobilized antigen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: sodium voltage-gated channel beta subunit 2
• Database Link:
  NP_037009
  Entrez Gene 6327 HumanEntrez Gene 72821 MouseEntrez Gene 25349 Rat
  UniProt ID P54900
• Background: Voltage-gated sodium channels (NaV) are essential for the generation of action potentials and for cell excitability. To date, nine NaV α subunits have been cloned and named NaV1.1-NaV1.9.2,3 Mammalian sodium channels are heterotrimers, composed of a central, pore-forming α subunit and two auxiliary β-subunits (NaVβ-subunit). The NaV β-subunit gene family consists of four members: β1 (SCN1B), β2 (SCN2B), β3 (SCN3B), and β4 (SCN4B) having type I topology, containing an extracellular amino-terminus, a single transmembrane segment, and an intracellular carboxyl-terminus.They modulate channel gating, assembly, and cell surface expression in heterologous cell systems. NaV β-subunits are cell adhesion molecules of the Ig superfamily, which interact with extracellular matrix, transmembrane signaling, and cell adhesion molecules. In the adult central nervous system and heart, sodium channels are associate with β1- β4 subunits, whereas in adult skeletal muscle they are associate only with the β1 subunit. This association appears to be a late event in sodium channel biosynthesis. Expression of β2-subunits increases in sensory neurons following nerve injury. β2 deficient mice develop less mechanical allodynia in compare to wild type mice, suggesting a role for β2 subunit in pain sensation.
• Synonyms: SCN2B