DIABLO Rabbit Polyclonal Antibody

CAT#: TA336333

Rabbit Polyclonal SMAC/Diablo Antibody

Product Images

Western Blot: SMAC/Diablo Antibody TA336333 - Analysis of Smac (DIABLO) in HeLa lysate using Smac antibody at 2 ug/ml.

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  Immunohistochemistry: SMAC/Diablo Antibody TA336333 - Analysis of human prostate using Smac antibody at 10 ug/ml.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Immunohistochemistry, Western Blot: 0.5-2 ug/ml, Immunocytochemistry/ Immunofluorescence: 1:10-1:500, Immunohistochemistry-Paraffin: 10 ug/ml
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: This rabbit polyclonal antibody was developed against two synthetic peptides corresponding to amino acids 199 to 212 and amino acids 226 to 239 of human Smac (Genbank accession no. AAF87716).
• Formulation: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: diablo IAP-binding mitochondrial protein
• Database Link:
  NP_620308
  Entrez Gene 56616 Human
  UniProt ID Q9NR28
• Background: Recently, three different laboratories have identified a molecule, Smac/DIABLO, which binds with inhibitor of apoptosis proteins (IAPs) and neutralizes IP inhibition of effector caspases. Multiple IAP molecules, including c-IAP1, c-IAP2, XIAP, and survivin are able to bind Smac. Smac/DIABLO is a mitochondrial protein, which is released into cytosol during apoptosis. The function of Smac is similar to that of Drosophila apoptosis activating proteins Reaper, Hid and Grim, which have been proposed to function by neutralizing the activity of IAPs. Smac promotes caspase-3 and caspase-9 activation by removing inhibitors rather than directly acting on Apaf-1, procaspases, and cytochrome c. The full-length cDNA of Smac encodes an open reading frame of 239 amino acids, predicting a protein with a molecular weight of 27 kDa. However, the mature protein purified from HeLa cells is only about 25 kDa.
• Synonyms: DFNA64; SMAC
• Note: Immunocytochemistry/Immunofluorescence: See Hu et al (2004) for details. Immunohistochemistry-Paraffin:10 ug/ml please see Ren et al. for details (2006).

Reference Data

• Protein Families: Transmembrane