CREB1 Mouse Monoclonal Antibody [Clone ID: 82B514.2]

CAT#: TA336386

Mouse Monoclonal CREB Antibody (82B514.2)

Product Images

Western Blot: CREB Antibody (82B514.2) TA336386 - Analysis of CREB in HeLa cell lysate using this antibody at 2 ug/ml.

Specifications

Product Data

• Clone Name: 82B514.2
• Applications: WB
• Recommended Dilution: WB: 2-4 ug/ml
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: A portion of amino acids 100-150 of human CREB was used as the immunogen.
• Formulation: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: cAMP responsive element binding protein 1
• Database Link:
  NP_004370
  Entrez Gene 1385 Human
  UniProt ID P16220
• Background: The transcription factor cAMP response element binding (CREB) binds the palindromic octanucleotide TGACGTCA (8,9). Members of ATF/CREB family include CREB-1, CREB-2 (also designated ATF-4), ATF-1, ATF-2 and ATF-3 (3-7). These proteins share highly conserved leucine zipper dimerization and basic DNA binding domains at the COOH terminal but are highly divergent in their amino terminal domains (5). CREB-1 is phosphorylated at Ser133, a key regulatory site controlling transcriptional activity, by many different kinases including protein kinase A, the calmodulin-dependent protein kinase CaMKII, p44/42 MAP kinase, p90RSK, p38 MAP kinase and MSK1 (2). Phosphorylation allows recruitment of CREB-binding protein (CBP), a large co-activator that contacts the general transcriptional machinery. CREB-mediated transcription regulates diverse cellular responses, including intermediary metabolism, neuronal signaling, cell proliferation, and apoptosis (1).
• Synonyms: CREB

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Antigen processing and presentation, Huntington's disease, Melanogenesis, Prostate cancer