Caspase-7 (CASP7) Mouse Monoclonal Antibody [Clone ID: 25B881.1]

CAT#: TA336390

Mouse Monoclonal Caspase-7 Antibody (25B881.1)

Product Images

Immunohistochemistry-Paraffin: Caspase-7 Antibody (25B881.1) TA336390 - Human tonsil stained with Caspase-7 antibody at 5 ug/ml. Novus' human tonsil tissue slide was used for this test. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

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  Western Blot: Caspase-7 Antibody (25B881.1) TA336390 - Analysis of Caspase-7 in A) human brain, B) mouse brain and C) rat brain lysate using Caspase-7 antibody at 1 ug/ml.

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  Western Blot: Caspase-7 Antibody (25B881.1) TA336390 - Analysis of Caspase-7 in Jurkat cells using Caspase-7 antibody at 1 ug/ml. Cells were treated with 2 uM staurosporine for different time periods. Caspase-7 activation is detected in western blots by the presence of Caspase-7 cleavage fragments. The antibody detected both pro (full-length) and active (cleaved) Caspase-7, depending on the treatment time points. A basal level of endogenous intermediate Caspase-7 can be see in untreated Jurkat cells.

Specifications

Product Data

• Clone Name: 25B881.1
• Applications: IHC, WB
• Recommended Dilution: Immunohistochemistry, Western Blot: 1-2 ug/ml, Immunohistochemistry-Paraffin: 5 ug/ml
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: This antibody was generated against recombinant protein of active form of human Caspase-7.
• Formulation: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: caspase 7
• Database Link:
  NP_203124
  Entrez Gene 12369 MouseEntrez Gene 64026 RatEntrez Gene 840 Human
  UniProt ID P55210
• Background: Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Caspase-7 is derived from the cleavage of proMCH3-alpha into 2 subunits, p20 and p12 by Caspase-3. Sequence analysis revealed that the MCH3-alpha amino acid sequence had the highest homology to caspase-3 (53% identity) and less than 37% identity with all other family members.
• Synonyms: CASP-7; CMH-1; ICE-LAP3; LICE2; MCH3

Reference Data

• Protein Families: Druggable Genome, Protease
• Protein Pathways: Alzheimer's disease, Apoptosis