PGP9.5 (UCHL1) Chicken Polyclonal Antibody

CAT#: TA336532

Chicken Polyclonal PGP9.5 / UCHL-1 Antibody

Product Images

Western Blot: PGP9.5 / UCHL-1 Antibody TA336532 - Blots of whole cell homogenate of the human SH-SY5Y neuroblastoma cell line stained with chicken antibody to glyceraldehyde 3 phosphate dehydrogenase (GAPDH, left lane, blot made with monoclonal antibod

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  Immunocytochemistry/Immunofluorescence: PGP9.5 / UCHL-1 Antibody TA336532 - Shows rat mixed neuron/glial cultures stained with chicken UCHL1 (green) and rabbit antibody to glial fibrillary acidic protein (GFAP-red), NB300-141. Blue is a DNA stain. Note

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 1:10000, IF: 1:1000
• Reactivities: Human, Rat
• Host: Chicken
• Isotype: IgY
• Clonality: Polyclonal
• Immunogen: Recombinant full length human UCHL1 purified from E. coli. [UniProt# P09936]
• Formulation: PBS, 0.03% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Ammonium sulfate precipitation
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 27 kDa
• Gene Name: ubiquitin C-terminal hydrolase L1
• Database Link:
  NP_004172
  Entrez Gene 29545 RatEntrez Gene 7345 Human
  UniProt ID P09936
• Background: UCH-L1 (ubiquitin carboxyl-terminal hydrolase isozyme L1) was the first discovered de-ubiquitinating enzyme which implicates in processing of ubiquitin precursors and also of ubiquitinated proteins. UCH-L1 is a thiol protease that recognizes and hydrolyzes a peptide bond at C-terminal glycine of ubiquitin. It also binds to free monoubiquitin and prevents its degradation in lysosomes. Localized in cytoplasm and ER membrane as lipid-anchor, UCH-L1 expression is restricted to brain, peripheral nerves, endocrine tissues and gonads of both sexes etc. UCH-L1 deletion in mice leads to fatal neurodegenerative disorder known as gracile axonal dystrophy and it is down-regulated in brains from Parkinson as well as Alzheimer disease patients. Expression outside of neuro-endocrine tissues is found in various cancers including B-cell lymphoma, multiple myeloma, and lung cancer. In transgenic mouse model, UCH-L1 has been demonstrated as an oncogene that causes malignancies by boosting AKT signalling. Furthermore, UCH-L1 has been shown to interfere with ubiquitination of RAPTOR which is catalyzed by DDB1-Cul4 E3 ligase complex, leading to loss of mTORC1 integrity accompanied by a concurrent increase in mTORC2, likely due to increased availability of free mTOR.
• Synonyms: HEL-117; NDGOA; PARK5; PGP 9.5; PGP9.5; PGP95; Uch-L1
• Note: This UCHL1 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blotting, where a band is seen at ~27 kDa.Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24190886)

Reference Data

• Protein Families: Druggable Genome, Protease
• Protein Pathways: Parkinson's disease