Tnk1 Rabbit Polyclonal Antibody

CAT#: TA336625

Rabbit Polyclonal Kos1/Tnk1 Antibody

Product Images

Western Blot: Kos1/Tnk1 Antibody TA336625 - T47D cells expressing GFP/GFP-Kos were lysed in RIPA lysis buffer. 500ug protein from clarified cell lysates was immunoprecipitated with an anti-GFP antibody. The washed immunoprecipitates were electrophoresed

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  Immunocytochemistry/Immunofluorescence: Kos1/Tnk1 Antibody TA336625 - Kos1 staining in Hela cells detected with a Dylight 488 labeled secondary antibody.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 1:500-1:1000, IF: 1:50-1:200
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: C-terminal residues 421-434 [QNQVQSLPAGSAEP] of the 47 kDa mouse Kos1 / Tnk1 protein.
• Formulation: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: tyrosine kinase, non-receptor, 1
• Database Link:
  NP_114086
  Entrez Gene 8711 HumanEntrez Gene 83813 Mouse
  UniProt ID Q99ML2
• Background: Kinase of embryonic stem cells (Kos1) is a nonreceptor protein tyrosine kinase (NRPTK) and a new member of the Ack family of NRPTKs. Kos1 is a ubiquitously expressed 47-kDa protein with autotyrosine kinase activity that is developmentally regulated during embryogenesis. Kos1 is also upregulated following IL3 withdrawal from factor-dependent murine NSF/N1.H7 cells that undergo apoptosis, suggesting a role in growth inhibition. Data support a negative regulatory role for Kos1 in regulating the Ras-Raf1-MAPK growth pathway by a mechanism that requires its autotyrosine kinase activity.
• Synonyms: MGC46193
• Note: This antibody may be used for Western blot and immunocytochemistry/immunofluorescence.