xCT (SLC7A11) Rabbit Polyclonal Antibody

CAT#: TA336629

Rabbit Polyclonal xCT Antibody


USD 457.00

5 Days*

Size
    • 100 ul

Frequently bought together (1)
beta Actin Mouse Monoclonal Antibody, Clone OTI1, Loading Control
    • 30 ul

USD 159.00

Specifications

Product Data
Applications WB
Recommended Dilution Simple Western, Immunohistochemistry: 1:200, Immunohistochemistry-Paraffin: 1:200, Western Blot: 0.5-2 ug/ml, Flow Cytometry: 1 - 5 ug/ml, Immunocytochemistry/ Immunofluorescence: 1:100 - 1:1000, Immunohistochemistry-Frozen: 1:10-1:500, Immunoblotting
Reactivities Human
Host Rabbit
Clonality Polyclonal
Immunogen A synthetic peptide made to a region within the N-terminus of the mouse xCT protein (between residues 1-50). [UniProt# Q9WTR6]
Formulation Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration lot specific
Purification Immunogen affinity purified
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Predicted Protein Size 55 kDa
Gene Name solute carrier family 7 member 11
Background System xc- is a cystine-glutamate exchange transporter that is composed of a light-chain subunit (xCT, SLC7A11) as well as a heavy-chain subunit (CD98hc, SLC3A2), and the xCT expression levels correlate with system xc-activity. SLC7A11 gene encodes xCT which is a multipass membrane protein with 12 transmembrane domains having both termini located intracellularly. xCT is widely expressed in various tissues and exert multiple roles such as pheomelanin production, cellular proliferation/metastasis, Kaposi's sarcoma herpesvirus (KSHV) entry into the host cells, learning and memory. Because cystine absorbed by cells via system xc- is rapidly reduced to cysteine for incorporation into GSH, the cell surface xCT expression is considered an important determinant of intracellular redox balance. Most cells require little cystine uptake to sustain cellular protein biosynthesis and GSH production, however this is different in cancer cells which generate high ROS levels via enhanced metabolism and require high GSH levels to survive oxidative stress. Accordingly, xCT is expressed at high levels in various malignant tumors including leukemias, lymphomas, Karposi's sarcoma, pancreatic cancer, and brain cancer.
Synonyms CCBR1; xCT
Note This xCT antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot. In Western blot a band is observed at ~55 kDa; in ICC/IF membrane and ER staining was visualized in HepG2 cells.
Reference Data
Protein Families Druggable Genome, Transmembrane

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.