Neurofilament (NEFL) Chicken Polyclonal Antibody

CAT#: TA336713

Chicken Polyclonal NF-L Antibody

Product Images

Western Blot: 68kDa Neurofilament Light Antibody TA336713 - Western blots of whole protein extracts of rat spinal cord (Lanes 1), brain stem (Lanes 2), cerebellum (Lanes 3) and cerebral cortex (Lanes 4). Neurofilaments are concentrated in large projecti

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  Immunocytochemistry/Immunofluorescence: 68kDa Neurofilament Light Antibody TA336713 - View of mixed neuron/glial cultures stained with TA336713 (red) and rabbit antibody to phosphorylated NF-H (NB300-135, green). The NF-L protein is assembled into neur

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 1:10000, IF: 1:5000
• Reactivities: Rat
• Host: Chicken
• Isotype: IgY
• Clonality: Polyclonal
• Immunogen: Neurofilament Light protein prepared from bovine spinal cord [UniProt# P02548]
• Formulation: PBS, 0.03% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Ammonium sulfate precipitation
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 68 kDa
• Gene Name: neurofilament, light polypeptide
• Database Link:
  NP_006149
  Entrez Gene 83613 Rat
  UniProt ID P07196
• Background: Neuronal intermediate filaments or neurofilaments are found specifically in neurons where they play a key role in maintaining neuronal morphology as well as in regenerating myelinated axons. Neurofilaments contain three subunits: a light polypeptide (NEFL/NFL), a medium polypeptide (NEFM/NFM), and a heavy polypeptide (NEFH/NFH), with molecular weights of 68, 160, and 212 kD respectively. 68kD Neurofilament Light (NEFL) is the most abundant of three neurofilament proteins and, like other nonepithelial intermediate filament proteins, it can form homopolymeric 10-nm filaments. NEFL forms the backbone of neurofilament and perturbations in its expression leads to motor neuron diseases, such as Charcot-Marie-Tooth disease, type 2E (CMT2E). In addition to its influence on the nervous system, NEFL has been shown to act as a tumor suppressor and has been linked to carcinogenesis/metastasis of several malignancues including breast, prostate, lung, colon, and bladder cancer.
• Synonyms: CMT1F; CMT2E; NF-L; NF68; NFL; PPP1R110
• Note: This 68kDa Neurofilament Light antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band can be seen at ~68 kDa.

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS
• Protein Pathways: Amyotrophic lateral sclerosis (ALS)