CD57 (B3GAT1) Rabbit Polyclonal Antibody

CAT#: TA336743

Rabbit Polyclonal beta-1,3-Glucuronyltransferase 1/B3GAT1/CD57 Antibody

Product Images

Immunocytochemistry/Immunofluorescence: beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody TA336743 - Antibody was tested in U2OS cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

• 

  Immunohistochemistry: beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody TA336743 - Analysis in mouse brain using DAB with hematoxylin counterstain.

Specifications

Product Data

• Applications: IHC
• Recommended Dilution: Immunohistochemistry: 1:100, Immunocytochemistry/ Immunofluorescence: 1:100, Immunohistochemistry-Paraffin: 1:400
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A synthetic peptide made to an N-terminal portion of the human CD57 protein (between residues 1-50) [UniProt Q9P2W7]
• Formulation: PBS with 30% Glycerol, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: beta-1,3-glucuronyltransferase 1
• Database Link:
  NP_473366
  Entrez Gene 76898 MouseEntrez Gene 117108 RatEntrez Gene 27087 Human
  UniProt ID Q9P2W7
• Background: CD57 (also known as beta-1,3-glucuronyltransferase 1, Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1, Glucuronosyltransferase P, Human Natural Killer 1, B3GAT1, NK1, HNK1, LEU7, NK-1, GLCUATP, and GLCATP) is a member of the glucuronyltransferase gene family. The enzymes produced by the members of this family recognize non-reducing terminal sugars and their anomeric linkages by exhibiting strong acceptor specificity. CD57 is also involved in the biosynthesis of glycosaminoglycan and L2/HNK-1 carbohydrate epitopes on glycoproteins. CD57 is expressed mainly in the brain and several alternate transcriptional splice variants have been characterized. In cancer research, CD57 positive lymphocytes have been used successfully in monitoring the progression of various cancers (PMID: 21491089). HNK1 is also involved in synaptic plasticity and neurodevelopment (PMID: 20950796).
• Synonyms: CD57; GLCATP; GLCUATP; HNK1; LEU7; NK-1; NK1
• Note: Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Reference Data

• Protein Families: Transmembrane
• Protein Pathways: Chondroitin sulfate biosynthesis, Heparan sulfate biosynthesis, Metabolic pathways