PGAM1 Rabbit Polyclonal Antibody

CAT#: TA336785

Rabbit Polyclonal PGAM1 Antibody

Product Images

Western Blot: PGAM1 Antibody TA336785 - Analysis of PGAM1 in Jurkat whole cell extracts

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  Immunocytochemistry/Immunofluorescence: PGAM1 Antibody TA336785 - Analysis of PGAM1 in HeLa cells using TA336785. Nuclei (Blue) are counterstained using Hoechst 33258.

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  Simple Western: PGAM1 Antibody TA336785 - Simple Western lane view shows a specific band for PGAM1 in 0.05 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

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  Immunohistochemistry: PGAM1 Antibody TA336785 - Analysis of PGAM1 in mouse tongue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Simple Western: 1:400, Immunocytochemistry/ Immunofluorescence: 1:50, Western Blot: 0.5ug/ml, Immunohistochemistry-Paraffin: 1:300, Immunohistochemistry: 1:300, Knockdown Validated
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A synthetic peptide made to a C-terminal portion of the human PGAM1 protein (between residues 200-254) [UniProt P18669]
• Formulation: PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: phosphoglycerate mutase 1
• Database Link:
  NP_002620
  Entrez Gene 18648 MouseEntrez Gene 5223 Human
  UniProt ID P18669
• Background: PGAM1 (phosphoglycerate mutase 1) is an enzyme that regulates a unique step in glycolysis, and most of the glycolytic intermediates that are used as precursors for anabolic biosynthesis are upstream of this step. PGAM1 catalyzes the interconversion of 3- phosphoglycerate (3-PGA) to 2- phosphoglycerate (2-PGA), and becomes activated or "primed" by an intrinsic phosphatase activity that converts 2,3-bisphosphoglycerate (2, 3-BPGA) to 2-PGA, phosphorylating the active site histidine in the process. The phosphohistidine is essential to carryout the mutase reaction, converting 3-PGA to 2-PGA in glycolysis. Interestingly, PGAM1 is the rate-limiting enzyme of glycolysis in tumor cells, heart tissue, and leukocytes, and its inhibition by epoxide inhibitors is lethal to cancer cells. In many cancers, including hepatocellular carcinoma and colorectal cancer, PGAM1 activity is increased compared to that in the normal tissues, wherein its expression is upregulated through loss of TP53 as TP53 negatively regulates PGAM1 gene expression.
• Synonyms: HEL-S-35; PGAM-B; PGAMA
• Note: This PGAM1 antibody may be used in Western blot, Immunohistochemistry paraffin embedded sections and Immunocytochemistry/Immunofluorescence.

Reference Data

• Protein Pathways: Glycolysis / Gluconeogenesis, Metabolic pathways