TIE2 (TEK) Rabbit Polyclonal Antibody

CAT#: TA336811

Rabbit Polyclonal Tie-2 Antibody


USD 424.00

5 Days*

Size
    • 100 ul

Product Images

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Specifications

Product Data
Applications IHC, WB
Recommended Dilution Western Blot: 1:2500, Immunohistochemistry-Paraffin: 1:200, Simple Western: 1:50, Immunohistochemistry: 1:200
Reactivities Human, Mouse
Host Rabbit
Clonality Polyclonal
Immunogen A genomic peptide made to an internal region of the human TIE2 protein (within residues 250-400). [Swiss-Prot Q02763]
Formulation PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration lot specific
Purification Immunogen affinity purified
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Gene Name TEK receptor tyrosine kinase
Background TIE2 (tyrosine-protein kinase receptor TIE-2) is an endothelial-selective RTK that is essential to angiopoietin (ANGPT)/TIE2 system which constitutes transmembrane endothelial tyrosine kinase TIE2 and its circulating ligands (ANGPT-1, -2 and ANGPT-3, -4). Activation/phosphorylation state of TIE2 regulates the baseline endothelial quiescence and its response to pathogens as well as cytokines, and TIE2 phosphorylation is largely controlled by the ratio of agonistic ligand ANGPT-1 and competitive inhibitor ANGPT-2. TIE2 exists as homodimer or heterodimer (with TIE1) and interacts with ANGPT1, ANGPT2, ANGPT4, TEK , TNIP2, SHC1, PTPRB, DOK2, GRB2, GRB7, GRB14, PIK3R1 and PTPN11/SHP2 etc for exerting its diverse biological functions. TIE2 regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion, actin cytoskeleton reorganization, and maintenance of vascular quiescence. It exerts anti-inflammatory effects by preventing the leakage of proinflammatory plasma proteins and leukocytes from blood vessels, and activates or inhibits angiogenesis, depending on the context (inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts). Targeted deletion of TIE2 or its major agonist ligand, Angpt-1, results in embryonic lethality in mice characterized by defects in blood vessel maturation, lack of recruitment of supporting pericytes and impaired basement membrane formation.
Synonyms CD202B; TIE-2; TIE2; VMCM; VMCM1
Note This TIE2 antibody is useful for Western blot where a band is seen ~150 kDa, and Immunohistochemistry-paraffin embedded sections where membrane, cytoplasmic and nuclear signal is seen in mouse intestines. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Reference Data
Protein Families Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase, Transmembrane

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.