HDAC5 Rabbit Polyclonal Antibody

CAT#: TA336929

Rabbit Polyclonal HDAC5 Antibody

Product Images

Western Blot: HDAC5 Antibody TA336929 - WB analysis of HDAC5 in 1. HeLa, 2. Ntera 2, 3. HepG2 and 4. MCF7 cell lysates.

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  Immunocytochemistry/Immunofluorescence: HDAC5 Antibody TA336929 - HDAC5 antibody was tested in A431 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

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  Immunohistochemistry: HDAC5 Antibody TA336929 - IHC analysis of HDAC5 in mouse pancreas.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Chromatin Immunoprecipitation, Western Blot: 1.0 - 2.0 ug/ml, Immunohistochemistry-Paraffin: 1:50 - 1:100, Immunohistochemistry: 1:50 - 1:100, Immunocytochemistry/ Immunofluorescence: 1:100 - 1:200, Chromatin Immunoprecipitation (ChIP)
• Reactivities: Human, Mouse, Primate
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A synthetic peptide made to an N-terminal portion of the human HDAC5 protein (between residues 1-100) [UniProt Q9UQL6]
• Formulation: PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 130 kDa
• Gene Name: histone deacetylase 5
• Database Link:
  NP_001015053
  Entrez Gene 15184 MouseEntrez Gene 10014 Human
  UniProt ID Q9UQL6
• Background: HDAC5, or histone deacetylase mHDA1, is responsible for the deacetylation of lysine residues on the N-terminal end of the core histones H2A, H2B, H3 and H4. Identifying histone acetyltransferases reveals much needed insight on how these enzymes regulate transcription, and further elucidates their pivotal role in cell cycle progression and other developmental events. HDAC5 shuttles between the cytoplasm and nucleus, depending on its nuclear export sequence, and shares interactions with BAHD1, KDM5B and MEF family proteins. While HDAC5 has many biological implications, recent studies have proposed its key involvement in synaptic plasticity and memory formation (PMID: 22914591), as well as its connection to various cancers (PMID: 22301920, PMID: 22944197).
• Synonyms: HD5; NY-CO-9
• Note: This HDAC5 antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry on paraffin embedded sections. In Western blot a band was observed ~ 130 kDa. In ICC/IF nuclear staining was observed in A431 cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Reference Data

• Protein Families: Druggable Genome, Transcription Factors