RIP3 (RIPK3) Rabbit Polyclonal Antibody

CAT#: TA337010

Rabbit Polyclonal RIP3/RIPK3 Antibody

Product Images

Western Blot: RIPK3/RIP3 Antibody TA337010 - Analysis of RIP3 using polyclonal RIPK3/RIP3 antibody TA337010. Human tissue blot probed with 5 ug/ml of RIP3 antibody: 1) brain, 2) heart, 3) small intestine, 4) kidney, 5) liver, 6) lung, 7) skeletal muscle, 8) stomach, 9) spleen, 10) ovary, 11) testis, 12) placenta probed at 5 ug/ml, 13) Ramos cell lysate probed at 7 ug/ml. Theoretical molecular weight 56.7 kDa.

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  Immunocytochemistry/Immunofluorescence: RIPK3/RIP3 Antibody TA337010 - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with RIPK3/RIP3 Antibody TA337010 at a 1:200 dilution overnight at 4C and detected with DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin was used at a 1:1000 dilution as a co-stain and detected with DyLight 550 (Red) at a 1:500 dilution. Nuclei were detected with DAPI (Blue) at 2.0ug/ml in 1X PBS. Cells were imaged using a 40X objective.

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  Immunohistochemistry-Paraffin: RIPK3/RIP3 Antibody TA337010 - Analysis of RIP3 using polyclonal RIPK3/RIP3 Antibody TA337010. Human pancreas probed with RIPK3/RIP3 antibody at 10 ug/ml.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Immunohistochemistry, Immunocytochemistry/ Immunofluorescence: 1:20-1:500, Immunohistochemistry-Paraffin: 10 ug/ml, Western Blot: 3-7 ug/ml, Immunoprecipitation
• Reactivities: Human, Primate
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A synthetic peptide corresponding to amino acids 497-522 (isoform CRA_a, 518 amino acids) and 298-335 (isoform CRA_b, 319 amino acids) of human RIP3 was used as immunogen for this antibody.
• Formulation: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at -20C. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Peptide affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: receptor interacting serine/threonine kinase 3
• Database Link:
  NP_006862
  Entrez Gene 11035 Human
  UniProt ID Q9Y572
• Synonyms: RIP3

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Cytosolic DNA-sensing pathway