TLR4 Rabbit Polyclonal Antibody

CAT#: TA337068

Rabbit Polyclonal TLR4 Antibody

Product Images

Flow Cytometry: TLR4 Antibody TA337068 - Cell surface analysis using TLR4 antibody. Mouse macrophage cells were probed using 1 ug of TLR4 antibody (red) and 1 ug of isotype control antibody (green). This antibody and PE-conjugated secondary were used for this test.

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  Immunocytochemistry/Immunofluorescence: TLR4 Antibody TA337068 - U2OS cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-TLR4 TA337068 at a 1:50 dilution overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

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  Flow Cytometry: TLR4 Antibody TA337068 - Analysis using the Alexa Fluor (R) 488 conjugate of TA337068. Staining of RAW cells were probed using 2 ug of TLR4 antibody (red) and 2 ug of isotype control antibody (green). Novus's TLR cell surface flow kit (10099K) was used for this test.

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  Western Blot: TLR4 Antibody TA337068 - Analysis of TLR4 using TLR4 antibody at 2 ug/mL on partial recombinant mouse TLR4 protein.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: Immunohistochemistry, Immunohistochemistry-Paraffin: 1:50, Immunocytochemistry/ Immunofluorescence: 1:50, Western Blot: 1 - 3 ug/mL, Flow Cytometry: 1 ug / 10^6 cells, Immunohistochemistry-Frozen: 1:10 - 1:500
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This antibody was developed against a sythetic peptide corresponding to amino acids 420-456 of human TLR4.
• Formulation: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at -20C. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: toll like receptor 4
• Database Link:
  NP_612564
  Entrez Gene 21898 MouseEntrez Gene 7099 Human
  UniProt ID O00206
• Synonyms: ARMD10; CD284; TLR-4; TOLL

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Pathogenic Escherichia coli infection, Toll-like receptor signaling pathway