Estrogen Receptor 1 (ESR1) Mouse Monoclonal Antibody

CAT#: TA347123

Mouse Monoclonal ER alpha Antibody

Product Images

WB analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with the antibody against ER alpha at a concentration of 7 ug/ml. Image shows the specificity of the antibody for the ER alpha isoform, whereas the ER beta isoform is not recognized.

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  Immunocytochemistry using the antibody against hERa COS-7 cells transiently overexpressing human hERa (left) or ERβ1 (right) were labeled with the antibody against ER alpha, used at a concentration of 15 ug/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Image shows the specificity of the antibody for the ER alpha isoform.

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  ChIP assays using MCF7 cells treated with ER agonist estradiol for 3 hr prior to harvesting. Sheared chromatin from 3 million cells and 5 ug of antibody were used per ChIP/input) and occupancy (x fold: +ve/-ve) are shown. qPCR primers were for the GREB1 promoter and for exon 2 of the myoglobin gene as negative control. Image shows recovery (the relative amount of IP'd DNA compared to input DNA) & occupancy (ratio +/- control target): demonstrate the occupancy of the GREB1 promoter by ERalpha.

Specifications

Product Data

• Applications: Assay, IHC, WB
• Recommended Dilution: ChIP (5 ug/IP) ; Western blotting (7 ug/ml); Immunochemistry (15 ug/ml)
• Reactivities: Human
• Host: Mouse
• Isotype: IgG3
• Clonality: Monoclonal
• Immunogen: The immunogen for anti-ER alpha antibody: the NH2 terminus of the human ER. (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).
• Concentration: lot specific
• Purification: Monoclonal antibody in PBS containing 0.05% azide; purified by ammonium sulphate precipitation followed by dialysis.
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: estrogen receptor 1
• Database Link:
  NP_000116
  Entrez Gene 2099 Human
• Synonyms: ER; Era; ESR; ESRA; ESTRR; NR3A1

Reference Data

• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors