KDM5B Rabbit Polyclonal Antibody

CAT#: TA347230

Rabbit Polyclonal Jarid1b Antibody

Product Images

WB was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the antibody against mouse Jarid1b, diluted 1:500 in BSA/PBS-Tween. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest (expected size: 179 kDa) is indicated on the right.

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  Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody against mouse Jarid1b. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2, 200.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: ELISA (1:100 ?? 1:200) ; Western blotting (1:500)
• Reactivities: Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The immunogen for anti-Jarid1b antibody: mouse Jarid1b (Jumonji, AT rich interactive domain 1B), using a KLH-conjugated synthetic peptides containing an amino acid sequence from the central part of the protein.
• Concentration: lot specific
• Purification: Whole antiserum from rabbit containing 0.05% azide.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: lysine demethylase 5B
• Database Link:
  NP_006609
  Entrez Gene 10765 Human
  UniProt ID Q9UGL1
• Background: JARID1B (UniProt/Swiss-Prot entry Q9UGL1) is a histone demethylase which specifically demethylates mono-, di, and trimethylated lysine 4 of histone H3. It acts as a transcriptional repressor for the tumor suppressor genes BRCA1 and HOXA5 and has been associated with breast cancer development. In contrast, in melanoma JARID1B itself may function as a tumor suppressor.
• Synonyms: CT31; JARID1B; PLU-1; PLU1; PPP1R98; PUT1; RBBP2H1A

Reference Data

• Protein Families: Druggable Genome, Transcription Factors