KMT2B Rabbit Polyclonal Antibody

CAT#: TA347232

Rabbit Polyclonal Mll2 Antibody

Product Images

WB was performed on whole cell lysates from mouse embryonic stem cells (E14Tg2a) with the antibody against mouse Mll2, diluted 1:500 in BSA/PBS-Tween. The location of the N-terminal fragment of Mll2 is indicated on the right. Cells homozygous for the targeted conditional mll2 allele ( D Mll2) show a dramatic reduction of Mll2 protein after partial recombination, whereas Mll2 protein was detected in D Mll1, D Mll4 and WT E14Tg2a cells.

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  Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody against mouse Mll2. The wells were coated with the peptides used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:32, 500.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: ELISA (1:200 ?? 1:2,000) ; Western blotting (1:500)
• Reactivities: Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The immunogen for anti-Mll2 antibody: mouse Mll2 (Myeloid/lymphoid or mixed-lineage leukemia protein 2), using two KLH-conjugated synthetic peptides containing an amino acid sequence from the central and from the N-terminal part of the protein, respective
• Concentration: lot specific
• Purification: Whole antiserum from rabbit containing 0.05% azide.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: lysine methyltransferase 2B
• Database Link:
  NP_055542
  Entrez Gene 75410 Mouse
  UniProt ID Q9UMN6
• Background: Mll2 (UniProtKB/Swiss-Prot entry Q9UMN6) plays a role in H3K4 methylation and may therefore act as a transciptional regulator. Mll2 is essential for embryonic development and may also be important for hematopiesis. Further, Mll2 shows an elevated expression in solid tumor cell lines, and may be involved in cancer.
• Synonyms: CXXC10; HRX2; MLL1B; MLL2; MLL4; TRX2; WBP-7; WBP7

Reference Data

• Protein Families: Druggable Genome