SETD1B Rabbit Polyclonal Antibody

CAT#: TA347285

Rabbit Polyclonal Setd1b Antibody

Product Images

WB was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the antibody against mouse Setd1b, diluted 1:500 in BSA/PBS-Tween. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.

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  Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody against mouse Setd1b. The plates were coated with a mix of the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:8, 400.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: ELISA (1:100 ?? 1:500) ; Western blotting (1:500)
• Reactivities: Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The immunogen for anti-Setd1b antibody: mouse Setd1b (Set domain containing 1B), using a 3 KLH-conjugated synthetic peptides containing sequences from the central part of the protein.
• Concentration: lot specific
• Purification: Whole antiserum from rabbit containing 0.05% azide.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: SET domain containing 1B
• Database Link:
  NP_055863
  Entrez Gene 23067 Human
  UniProt ID Q9UPS6
• Background: SETD1B (UniProt/Swiss-Prot entry Q9UPS6) is a component of the SET1 histone methyltransferase (HMT) complex. This complex specifically methylates lysine 4 of histone H3, but only if the neighboring lysine 9 residue is not yet methylated. Methylation of H3K4 represents a specific tag for epigenetic transcriptional activation. SETD1B shows a non-overlapping localization with SETD1A, suggesting that both proteins play a specific role in the epigenetic control of chromatin structure and gene expression.
• Synonyms: KMT2G; Set1B

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Lysine degradation