SIRT1 Mouse Monoclonal Antibody

CAT#: TA347290

Mouse Monoclonal SirT1 Antibody

Product Images

WB was performed using nuclear extracts from HeLa cells (HeLa NE, 20 ug) and the antibody against hSirT1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left, the position of the protein of interest is shown on the right.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western blotting (1:1,000)
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: The immunogen for anti-SirT1 antibody: human SirT1 (NAD-dependent deacetylase sirtuin-1), using a synthetic peptide.
• Concentration: lot specific
• Purification: Ascites fluid from mouse containing 0.05% azide.
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: sirtuin 1
• Database Link:
  NP_036370
  Entrez Gene 23411 Human
• Background: SirT1 (UniProtKB/Swiss-Prot entry Q96EB6) is a NAD-dependent deacetylase, which regulates processes such as apoptosis and muscle differentiation by the deacetylation of key regulatory proteins. Deacetylation of lysine 382 of p53/TP53 by Sirt1 impairs its ability to induce the proapoptotic program and modulate cell senescence, whereas deacetylation of TAF1B represses rDNA transcription by the RNA polymerase I. SirT1 is also involved in HES1- and HEY2-mediated transcriptional repression and inhibits skeletal muscle differentiation by deacetylating PCAF and MYOD1. SirT1 is recruited to the nuclear bodies via its interaction with PML.
• Synonyms: SIR2; SIR2alpha; SIR2L1

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors