ME1 Rabbit Polyclonal Antibody

CAT#: TA349028

Rabbit Polyclonal Anti-ME2 Antibody

Product Images

Western blot analysis of ME2 in human spleen tissue lysate with ME2 antibody at (A) 1 and (B) 2ug/mL.

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  Immunohistochemistry of ME2 in human spleen tissue with ME2 antibody at 5 ug/mL.

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  Immunofluorescence of ME2 in human spleen tissue with ME2 antibody at 20 ug/mL.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1 - 2 ug/mL, IHC: 5 ug/mL, IF: 20 ug/mL
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: ME2 antibody was raised against a 17 amino acid peptide near the center of human ME2.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: ME2 antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: Predicted: 64 kDa; Observed: 60 kDa
• Gene Name: malic enzyme 1
• Database Link:
  NP_002386
  Entrez Gene 4199 Human
  UniProt ID P48163
• Background: ME2 is a homotetrameric, mitochondrial NAD-dependent malic enzyme that catalyzes the oxidative decarboxylation of malate to pyruvate (1). It is related to malic enzyme 1 (ME1), a cytoplasmic NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis (2). The expression of both malic enzymes is reciprocally regulated by p53; this regulation has been shown to modulate metabolism and senescence (3). Certain single-nucleotide polymorphism haplotypes of the ME2 gene have been shown to increase the risk for idiopathic generalized epilepsy (4).
• Synonyms: HUMNDME; MES

Reference Data

• Protein Pathways: Metabolic pathways, PPAR signaling pathway, Pyruvate metabolism