CD33 Rabbit Polyclonal Antibody

CAT#: TA349777

Rabbit Polyclonal Anti-CD33 Antibody

Product Images

Gel: 8%SDS-PAGE Lysate: 40 μg Lane 1-3: HepG2 cell Mouse liver tissue Jurkat cell lysates Primary antibody: TA349777 (CD33 Antibody) at dilution 1/750 Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution Exposure time: 3 minutes

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  Immunohistochemistry of paraffin-embedded Human gastric cancer tissue using TA349777 (CD33 Antibody) at dilution 1/30 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human gastric cancer tissue using TA349777 (CD33 Antibody) at dilution 1/30, treated with fusion protein. (Original magnification: ×200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: HepG2 cell, Mouse liver tissue, Jurkat cell lysates
  IHC: 50-100
  Positive control: Human gastric cancer
  Predicted cell location: Cytoplasm and Cell membrane
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein of human CD33
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glyceroln
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 40 kDa
• Gene Name: CD33 molecule
• Database Link:
  NP_001763
  Entrez Gene 12489 MouseEntrez Gene 945 Human
  UniProt ID P20138
• Background: CD33 or Siglec-3 is a transmembrane receptor expressed on cells of myeloid lineage. It is usually considered myeloid-specific, but it can also be found on some lymphoid cells. Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules.
• Synonyms: p67; SIGLEC-3; SIGLEC3

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Hematopoietic cell lineage