BCAR1 Rabbit Polyclonal Antibody

CAT#: TA350795

Rabbit Polyclonal Anti-BCAR1 Antibody

Product Images

Gel: 6%SDS-PAGE Lysate: 40 μg Lane 1-3: Hela cells 231 cells A431 cells Primary antibody: TA350795 (BCAR1 Antibody) at dilution 1/100 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 4 minutes

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  Immunohistochemistry of paraffin-embedded Human gasrtic cancer tissue using TA350795 (BCAR1 Antibody) at dilution 1/15 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human gasrtic cancer tissue using TA350795 (BCAR1 Antibody) at dilution 1/15, treated with synthetic peptide. (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA350795 (BCAR1 Antibody) at dilution 1/15 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA350795 (BCAR1 Antibody) at dilution 1/15, treated with synthetic peptide. (Original magnification: ×200)

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Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 200-1000
  WB positive control: Hela, 231 and A431 cells
  IHC: 25-100
  Positive control: Human gasrtic cancer
  Predicted cell location: Cytoplasm
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide of human BCAR1
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glyceroln
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 93 kDa
• Gene Name: BCAR1, Cas family scaffolding protein
• Database Link:
  NP_055382
  Entrez Gene 12927 MouseEntrez Gene 25414 RatEntrez Gene 9564 Human
  UniProt ID P56945
• Background: BCAR1, or CAS, is an Src (MIM 190090) family kinase substrate involved in various cellular events, including migration, survival, transformation, and invasion. The molecular cloning of p130 Cas has shown it to represent a novel SH3 containing signaling molecule with a cluster of multiple putative SH2-binding motifs for v-Crk. By immunoprecipitation analysis, p130 Cas has been shown to be highly phosphorylated at tyrosine residues subsequent to either v-Src p60 or v-Crk-mediated transformation and to form stable complexes with both of these transforming proteins. p130 Cas behaves as an extremely potent substrate for protein tyrosine kinases and has been reported to relocate from the cytoplasm to cell membrane upon tyrosine phosphorylation. One proposed model is that the SH2 domain of v-Crk functions to activate c-Src kinase, which in turn phosphorylates p130 Cas.
• Synonyms: CAS; CAS1; CASS1; CRKAS; P130Cas

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Chemokine signaling pathway, Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton