Glutaminase (GLS) Rabbit Polyclonal Antibody

CAT#: TA351221

Rabbit Polyclonal Anti-GLS Antibody

Product Images

Gel: 6%SDS-PAGE Lysate: 40 μg Lane: 293T cell lysate Primary antibody: TA351221 (GLS Antibody) at dilution 1/850 Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution Exposure time: 1 minute

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  Immunohistochemistry of paraffin-embedded Human liver cancer tissue using TA351221 (GLS Antibody) at dilution 1/70 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human liver cancer tissue using TA351221 (GLS Antibody) at dilution 1/70, treated with synthetic peptide. (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human tonsil tissue using TA351221 (GLS Antibody) at dilution 1/70 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human tonsil tissue using TA351221 (GLS Antibody) at dilution 1/70, treated with synthetic peptide. (Original magnification: ×200)

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Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: 293T cell lysate
  IHC: 50-200
  Positive control: Human liver cancer
  Predicted cell location: Cytoplasm
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide of human GLS
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glyceroln
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 73 kDa
• Gene Name: glutaminase
• Database Link:
  NP_055720
  Entrez Gene 14660 MouseEntrez Gene 24398 RatEntrez Gene 2744 Human
  UniProt ID O94925
• Background: This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants.
• Synonyms: AAD20; GAC; GAM; GLS1; KGA

Reference Data

• Protein Pathways: Alanine, aspartate and glutamate metabolism, Arginine and proline metabolism, D-Glutamine and D-glutamate metabolism, Metabolic pathways, Nitrogen metabolism