Bim (BCL2L11) Rabbit Polyclonal Antibody

CAT#: TA354317

Rabbit anti Bim Polyclonal Antibody

Product Images

IHC: Human placenta stained with Rabbit anti-BIM antibody, at 1:25 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

Specifications

Product Data

• Applications: IHC
• Recommended Dilution: WB 0.1-1 µg/ml ELISA 0.01-0.1 µg/ml IP 2-5 µg/ml IHC 2-10 µg/ml FC 5-10 µg/ml
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide derived from N-terminus of human BIM.
• Formulation: This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
• Purification: The Rabbit IgG is purified by Epitope Affinity Purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: ~32, 29 and 15 kDa
• Gene Name: BCL2 like 11
• Database Link:
  NP_001191035
  Entrez Gene 12125 MouseEntrez Gene 64547 RatEntrez Gene 10018 Human
  UniProt ID O43521
• Background: Bim (bcl-2-interacting mediator of cell death, BCL-2-related Ovarian Death Gene) is a proapoptotic member of the Bcl-2 family that shares the homology domain3 (BH3) with this family. Bim exists in three isoforms: BimEL, BimL, and BimS. As the BCL-2 family members reside upstream of irreversible cellular damage and focus much of their efforts at the level of mitochondria, they play a pivotal role in deciding whether a cell will live or die. In healthy cells, BIM molecules are localized to the microtubule-associated dynein motor complex through association with the LC8 dynein light chain. Following a death signal, LC8 and BIM dissociate from the motor complex and together translocate to the mitochondria. At the mitochondria, BIM is thought to interact with BCL-2 to antagonize its antiapoptotic activity.
• Synonyms: BAM; BIM; BOD

Reference Data

• Protein Families: Druggable Genome