p21 (CDKN1A) Mouse Monoclonal Antibody [Clone ID: SPM306]

CAT#: TA354354

Mouse anti p21 Monoclonal Antibody

Product Images

IHC: Human breast cancer tissue stained with Mouse Anti-p21antibody, at 1:50 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

Specifications

Product Data

• Clone Name: SPM306
• Applications: IHC
• Recommended Dilution: WB 0.1-1 µg/ml ELISA 0.01-0.1 µg/ml IP 2-5 µg/ml IHC 2-10 µg/ml FC 5-10 µg/ml
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant human p57/Kip2 protein.
• Formulation: This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
• Purification: The mouse IgG is purified by Protein A-Affinity Chromatography according to Isotyping
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: ~21 kDa
• Gene Name: cyclin-dependent kinase inhibitor 1A
• Database Link:
  NP_000380
  Entrez Gene 1026 Human
  UniProt ID P38936
• Background: p57/Kip 2 (or CDKN 1C) is a tight-binding strong inhibitor of several G1 cycline/Cdk complexes and a negative regulator of cell proliferation. The gene is a tumor suppressor which is located on chromosome 11p15.5. Mutations in this gene are implicated in sporadic cancers and Beckwith-Wiedemann syndrome (BWS). Several types of childhood tumors including Wilms' tumor, adrenocortical carcinoma and rhabdomyosarcoma display a specific loss of maternal 11p15 alleles, suggesting that genomic imprinting plays an important part. This region also contains two other imprinted genes, insulin like growth factor II (IGF II) and H19, both of which seem to be implicated in adrenal neoplasms.
• Synonyms: CAP20; CDKN1; CIP1; MDA-6; P21; p21CIP1; SDI1; WAF1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Bladder cancer, Cell cycle, Chronic myeloid leukemia, ErbB signaling pathway, Glioma, Melanoma, p53 signaling pathway, Pathways in cancer, Prostate cancer