alpha Tubulin (TUBA1A) Rabbit Polyclonal Antibody

CAT#: TA354999

Rabbit Polyclonal Anti-alpha-Tubulin Antibody

Product Images

Western blot analysis of alpha-Tubulin in 293, Daudi, Hela, HepG2, Jurkat, K562, NH3T3, Raji, Ramos, U937, Human brain, Mouse brain, Rat Brain, Rabbit Brain, Rabbit Spleen, Zebrafish, Mouse Liver and Chicken liver lysate at 1ug/mL.

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  Immunohistochemistry of alpha-Tubulin in human brain tissue with alpha-Tubulin antibody at 5ug/mL.

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  Immunohistochemistry of alpha-Tubulin in human brain tissue with alpha-Tubulin antibody at 2.5ug/ml.

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  Immunofluorescence of alpha-Tubulin in human brain tissue with alpha-Tubulin antibody at 20ug/mL.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1-2µg/ml.IHC starting at 2.5µg/mL. IF start at 20µg/mL.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: alpha-Tubulin antibody was raised against an 18 amino acid peptide near the carboxy terminus of human alpha-Tubulin
• Formulation: alpha-Tubulin antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: alpha-Tubulin antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: Predicted: 50 kDa; Observed: 54 kDa
• Gene Name: tubulin alpha 1a
• Database Link:
  NP_006000
  Entrez Gene 22142 MouseEntrez Gene 64158 RatEntrez Gene 7846 Human
  UniProt ID Q71U36
• Background: alpha-Tubulin belongs to the tubulin superfamily, which is composed of six distinct families. Along with beta-tubulins, alpha-Tubulins are the major components of microtubules. These microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Alpha- and beta-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter (reviewed in 1). Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha-Tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase (2). Another post-translational modification of detyrosinated alpha-Tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle (3). Like GAPDH and ?-Actin, this antibody makes an excellent loading control in immunoblots.
• Synonyms: B-ALPHA-1; FLJ25113; hum-a-tub1; hum-a-tub2; LIS3; TUBA3
• Note: alpha-Tubulin antibody can be used for detection of alpha-Tubulin by Western blot at 1 - 2 µg/ml. Antibody can also be used for Immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL.

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Gap junction, Pathogenic Escherichia coli infection