alpha Tubulin (TUBA1A) Mouse Monoclonal Antibody [Clone ID: 2B11]

CAT#: TA355046

Mouse Monoclonal Anti-alpha-Tubulin Antibody [2B11]

Product Images

Western blot of alpha-Tubulin in 293, A431, A549, Daudi, HeLa, HepG2, Jurkat, K562, MOLT4, 3T3, Raji, Ramos, THP-1, U937, human brain, mouse brain, rat brain, rabbit brain, mouse lung, rat lung, mouse liver, rabbit spleen and zebrafish lysate at 1ug/ml.

Specifications

Product Data

• Clone Name: 2B11
• Applications: WB
• Recommended Dilution: WB: 1-2µg/ml.
• Reactivities: Human, Mouse, Rabbit, Rat, Zebrafish
• Host: Mouse
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: alpha-Tubulin antibody was raised against an 18 amino acid peptide near the carboxy terminus of human alpha-Tubulin
• Formulation: alpha-Tubulin antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: alpha-Tubulin antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: Predicted: 50 kDa; Observed: 54 kDa
• Gene Name: tubulin alpha 1a
• Database Link:
  NP_006000
  Entrez Gene 22142 MouseEntrez Gene 64158 RatEntrez Gene 7846 Human
  UniProt ID Q71U36
• Background: Alpha-Tubulin belongs to the tubulin superfamily, which is composed of six distinct families. Along with beta-tubulins, alpha-Tubulins are the major components of microtubules. These microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Alpha- and beta-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter (reviewed in 1). Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha-Tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase (2). Another post-translational modification of detyrosinated alpha-Tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle (3). Like GAPDH and ?-Actin, this antibody makes an excellent loading control in immunoblots.
• Synonyms: B-ALPHA-1; FLJ25113; hum-a-tub1; hum-a-tub2; LIS3; TUBA3
• Note: alpha-Tubulin antibody can be used for detection of alpha-Tubulin by Western blot at 1 - 2 µg/ml.

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Gap junction, Pathogenic Escherichia coli infection