CD22 Mouse Monoclonal Antibody [Clone ID: IS7]

CAT#: TA363832

CD22 (B-lymphocyte cell adhesion molecule, Leu-14), mouse anti human, clone IS7

Product Images

TA363832, Clone IS7, human tonsil, frozen section

Specifications

Product Data

• Clone Name: IS7
• Applications: IHC
• Recommended Dilution: Tested for immunohistochemistry (IHC); has been described to work in FACS. Approximate working dilution for IHC: Frozen sections: 0.2-0.4µg/ml (1:500 - 1:1000) Paraffin sections: does not react on routinely processed paraffin sections. Optimal dilutions should be determined by the end user. Suggested positive control: Human tonsil
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Specificity: Human: CD22 Other: does not react with porcine tissues.
  
  Epitope: The antigen is CD22. The epitope has not been further characterized.
  
  Distribution: Isolated cells: 5-50% of human peripheral blood mononuclear cells. Tissue sections: The antigen is found on precursor and mature B-cells.
• Formulation: Affinity purified, lyophilized Reconstitute by adding 0.5ml distilled water. This stock solution contains 0.2mg/ml IgG, phosphate buffered saline pH 7.2 (PBS), 5mg/ml bovine serum albumin (BSA), and 0.09% sodium azide as a preservative.
• Concentration: N/A
• Conjugation: Unconjugated
• Storage: Original vial: 1 year at 4° - 8°C. Stock solution or aliquots thereof: 1 year at -20°C. Avoid repeated thawing and freezing.
• Gene Name: CD22 molecule
• Database Link:
  Entrez Gene 933 Human
  UniProt ID P20273
• Background: Monoclonal antibody IS7 recognizes the CD22 antigen also known as Leu-14, which is expressed on precursor and mature B-cells. CD22 is a type 1 integral membrane glycoprotein with a molecular weight of 130 to 140kD. It is expressed in both the cytoplasm and the cell membrane of B lymphocytes. The CD22 antigen appears early in B lymphocyte differentiation at approximately the same stage as the CD19 antigen. CD22 has been detected on approximately 5-50% of human peripheral blood mononuclear cells.
• Synonyms: BL-CAM; FLJ22814; Leu-14; MGC130020; Siglec-2; SIGLEC2