GCLC Rabbit Polyclonal Antibody

CAT#: TA365360

GCLC rabbit polyclonal antibody

Product Images

Gel: 6%SDS-PAGE Lysate: 40 μg Lane 1-4: Raw264.7 A549 Hepg2 and Jurkat cell Primary antibody: TA365360 (GCLC Antibody) at dilution 1/800 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 30 seconds

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  Immunohistochemistry of paraffin-embedded Human brain tissue using TA365360 (GCLC Antibody) at dilution 1/35 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human brain tissue using TA365360 (GCLC Antibody) at dilution 1/35, treated with fusion protein. (Original magnification: ×200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: Raw264.7，A549，Hepg2 and Jurkat cell
  IHC: 25-100
  Positive control: Human brain
  Predicted cell location: Cytoplasm and Cell membrane
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein of human GCLC
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C.
• Stability: 1 year
• Predicted Protein Size: 73 kDa
• Database Link:
  UniProt ID P48506
• Background: Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.