U2AF1 Rabbit Polyclonal Antibody

CAT#: TA365453

U2AF1 rabbit polyclonal antibody

Product Images

Gel: 8%SDS-PAGE Lysate: 40 μg Lane 1-2: 231 cell Rat liver tissue lysates Primary antibody: TA365453 (U2AF1 Antibody) at dilution 1/1350 Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution Exposure time: 30 seconds

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  Immunohistochemistry of paraffin-embedded Human liver cancer tissue using TA365453 (U2AF1 Antibody) at dilution 1/200 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human liver cancer tissue using TA365453 (U2AF1 Antibody) at dilution 1/200, treated with fusion protein. (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human gastric cancer tissue using TA365453 (U2AF1 Antibody) at dilution 1/200 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human gastric cancer tissue using TA365453 (U2AF1 Antibody) at dilution 1/200, treated with fusion protein. (Original magnification: ×200)

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Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: 231 cell, Rat liver tissue lysates
  IHC: 200-400
  Positive control: Human liver cancer
  Predicted cell location: Nucleus
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein of human U2AF1
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C.
• Stability: 1 year
• Predicted Protein Size: 28 kDa
• Database Link:
  UniProt ID Q01081
• Background: This gene belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Alternatively spliced transcript variants encoding different isoforms have been identified.