VPS35 Rabbit Polyclonal Antibody

CAT#: TA372057

VPS35 rabbit polyclonal antibody

Product Images

Gel: 6%SDS-PAGE Lysate: 40 μg Lane 1-7: Hela HEPG2 Raji NIH/3T3 and A549 cell lysates Human cerebella tissue and Mouse brain tissue lysates Primary antibody: TA372057 (VPS35 Antibody) at dilution 1/250 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 1 minute

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  Immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using TA372057 (VPS35 Antibody) at dilution 1/25 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using TA372057 (VPS35 Antibody) at dilution 1/25, treated with synthetic peptide. (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA372057 (VPS35 Antibody) at dilution 1/25 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA372057 (VPS35 Antibody) at dilution 1/25, treated with synthetic peptide. (Original magnification: ×200)

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Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: Hela,HEPG2,Raji,NIH/3T3 and A549 cell lysates,Human cerebella tissue and Mouse brain tissue lysates
  IHC: 20-100
  Positive control: Human colorectal cancer
  Predicted cell location: Cytoplasm or Nucleus
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide of human VPS35
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C.
• Stability: 1 year
• Predicted Protein Size: 92 kDa
• Gene Name: VPS35 retromer complex component
• Database Link:
  Entrez Gene 55737 Human
  UniProt ID Q96QK1
• Background: This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex.
• Synonyms: DKFZp434E1211; DKFZp434P1672; FLJ10752; FLJ13588; FLJ20388; hVPS35; MEM3