ACSS1 Rabbit Polyclonal Antibody

CAT#: TA373217

ACSS1 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using ACSS1 antibody (TA373217) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 10s.

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  Immunohistochemistry of paraffin-embedded Human colon carcinoma using ACSS1 Rabbit pAb (TA373217) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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  Immunofluorescence analysis of L929 cells using ACSS1 antibody (TA373217) at dilution of 1:100. Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:200
  IF,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 340-689 of human ACSS1 (NP_115890.2).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 62kDa/74kDa
• Gene Name: acyl-CoA synthetase short-chain family member 1
• Database Link:
  Entrez Gene 84532 Human
  UniProt ID Q9NUB1
• Background: This gene encodes a mitochondrial acetyl-CoA synthetase enzyme. A similar protein in mice plays an important role in the tricarboxylic acid cycle by catalyzing the conversion of acetate to acetyl CoA. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
• Synonyms: ACAS2L; AceCS2; AceCS2L; dJ568C11.3; FLJ45659; KIAA1846; MGC33843