ACTC1 Rabbit Polyclonal Antibody

CAT#: TA373223

ACTC1 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using ACTC1 antibody (TA373223) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 10s.

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  Immunohistochemistry of paraffin-embedded Human skeletal muscle using ACTC1 Rabbit pAb (TA373223) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human ACTC1.
• Formulation: Buffer: PBS with 0.02% sodium azide,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at 4℃. Avoid freeze / thaw cycles.|Buffer: PBS with 0.02% sodium azide,pH7.3.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 42kDa
• Gene Name: actin, alpha, cardiac muscle 1
• Database Link:
  Entrez Gene 70 Human
  UniProt ID P68032
• Background: Actins are highly conserved proteins that are involved in various types of cell motility. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to four others. The protein encoded by this gene belongs to the actin family which is comprised of three main groups of actin isoforms, alpha, beta, and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. Defects in this gene have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC).
• Synonyms: ACTC; ASD5; CMD1R; CMH11