alpha 2a Adrenergic Receptor (ADRA2A) Rabbit Polyclonal Antibody

CAT#: TA373321

ADRA2A Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using ADRA2A antibody (TA373321) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 30s.

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  Immunofluorescence analysis of C6 cells using ADRA2A Rabbit pAb (TA373321) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of HeLa cells using ADRA2A Rabbit pAb (TA373321) at dilution of 1:100. Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IF,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 240-380 of human ADRA2A (NP_000672.3).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 48kDa
• Gene Name: adrenoceptor alpha 2A
• Database Link:
  Entrez Gene 150 Human
  UniProt ID P08913
• Background: Alpha-2-adrenergic receptors are members of the G protein-coupled receptor superfamily. They include 3 highly homologous subtypes: alpha2A, alpha2B, and alpha2C. These receptors have a critical role in regulating neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system. Studies in mouse revealed that both the alpha2A and alpha2C subtypes were required for normal presynaptic control of transmitter release from sympathetic nerves in the heart and from central noradrenergic neurons; the alpha2A subtype inhibited transmitter release at high stimulation frequencies, whereas the alpha2C subtype modulated neurotransmission at lower levels of nerve activity. This gene encodes alpha2A subtype and it contains no introns in either its coding or untranslated sequences.
• Synonyms: ADRA2; ADRA2R; ADRAR; Alpha-2AAR; ALPHA2AAR; ZNF32