HYPE (FICD) Rabbit Polyclonal Antibody

CAT#: TA376252

FICD Rabbit polyclonal Antibody

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB,1:500 - 1:2000
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 119-458 of human FICD (NP_009007.2).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 51kDa
• Gene Name: FIC domain containing
• Database Link:
  Entrez Gene 11153 Human
  UniProt ID Q9BVA6
• Background: Protein that can both mediate the addition of adenosine 5'-monophosphate (AMP to specific residues of target proteins (AMPylation, and the removal of the same modification from target proteins (de-AMPylation, depending on the context (By similarity. The side chain of Glu-231 determines which of the two opposing activities (AMPylase or de-AMPylase will take place (By similarity. Acts as a key regulator of the ERN1/IRE1-mediated unfolded protein response (UPR by mediating AMPylation or de-AMPylation of HSPA5/BiP. In unstressed cells, acts as an adenylyltransferase by mediating AMPylation of HSPA5/BiP at 'Thr-518', thereby inactivating it (By similarity. In response to endoplasmic reticulum stress, acts as a phosphodiesterase by mediating removal of ATP (de-AMPylation from HSPA5/BiP at 'Thr-518', leading to restore HSPA5/BiP activity (By similarity. Although it is able to AMPylate RhoA, Rac and Cdc42 Rho GTPases in vitro, Rho GTPases do not constitute physiological substrates.