Myelin expression factor 2 (MYEF2) Rabbit Polyclonal Antibody

CAT#: TA378826

MYEF2 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using MYEF2 antibody (TA378826) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 30s.

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  Immunohistochemistry of paraffin-embedded Mouse brain using MYEF2 Rabbit pAb (TA378826) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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  Immunohistochemistry of paraffin-embedded Rat testis using MYEF2 Rabbit pAb (TA378826) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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  Immunohistochemistry of paraffin-embedded Human liver cancer using MYEF2 Rabbit pAb (TA378826) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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  Immunofluorescence analysis of C6 cells using MYEF2 antibody (TA378826) at dilution of 1:100. Blue: DAPI for nuclear staining.

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Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:200
  IF,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human MYEF2 (NP_001288139.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 14kDa/20kDa/61kDa/64kDa
• Gene Name: myelin expression factor 2
• Database Link:
  Entrez Gene 50804 Human
  UniProt ID Q9P2K5
• Background: Transcriptional repressor of the myelin basic protein gene (MBP. Binds to the proximal MB1 element 5'-TTGTCC-3' of the MBP promoter. Its binding to MB1 and function are inhibited by PURA (By similarity.
• Synonyms: FLJ11213; HsT18564; KIAA1341; MEF-2; MGC87325; MST156; MSTP156; MyEF-2