NDUFS8 Rabbit Polyclonal Antibody

CAT#: TA379025

NDUFS8 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using NDUFS8 antibody (TA379025) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 90s.

• 

  Immunofluorescence analysis of L929 cells using NDUFS8 Polyclonal Antibody (TA379025) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:100
  IF,1:50 - 1:100
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 35-210 of human NDUFS8 (NP_002487.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 23kDa
• Gene Name: NADH:ubiquinone oxidoreductase core subunit S8
• Database Link:
  Entrez Gene 4728 Human
  UniProt ID O00217
• Background: This gene encodes a subunit of mitochondrial NADH:ubiquinone oxidoreductase, or Complex I, a multimeric enzyme of the respiratory chain responsible for NADH oxidation, ubiquinone reduction, and the ejection of protons from mitochondria. The encoded protein is involved in the binding of two of the six to eight iron-sulfur clusters of Complex I and, as such, is required in the electron transfer process. Mutations in this gene have been associated with Leigh syndrome.
• Synonyms: CI-23kD; CI23KD; TYKY