Tropomyosin 2 (TPM2) Rabbit Polyclonal Antibody

CAT#: TA382756

TPM2 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using TPM2 antibody (TA382756) at 1:400 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 90s.

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  Immunohistochemistry of paraffin-embedded human stomach using TPM2 antibody (TA382756) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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  Immunohistochemistry of paraffin-embedded rat heart using TPM2 antibody (TA382756) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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  Immunohistochemistry of paraffin-embedded mouse heart using TPM2 antibody (TA382756) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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  Immunoprecipitation analysis of 300ug extracts of Mouse skeletal muscle cells using 3ug TPM2 antibody (TA382756). Western blot was performed from the immunoprecipitate using TPM2 antibody (TA382756) at a dilition of 1:1000.

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Specifications

Product Data

• Applications: IF, IHC, IP, WB
• Recommended Dilution: WB,1:500 - 1:1000
  IHC,1:50 - 1:100
  IF,1:50 - 1:100
  IP,1:50 - 1:100
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide of human TPM2
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 28kDa/32kDa
• Gene Name: tropomyosin 2 (beta)
• Database Link:
  Entrez Gene 7169 Human
  UniProt ID P07951
• Background: This gene encodes beta-tropomyosin, a member of the actin filament binding protein family, and mainly expressed in slow, type 1 muscle fibers. Mutations in this gene can alter the expression of other sarcomeric tropomyosin proteins, and cause cap disease, nemaline myopathy and distal arthrogryposis syndromes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Synonyms: AMCD1; Beta-tropomyosin; DA1; DA2B; NEM4; TMSB; Tropomyosin-2